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affigel protein a column (Bio-Rad)

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Bio-Rad affigel protein a column
Affigel Protein A Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+a+affigel+column/Affi-Gel+Protein+A+Support/us09637546-344-15-24
Average 93 stars, based on 303 article reviews

affigel protein a column - by Bioz Stars, 2026-09

93/100 stars

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Purification:

Article Title: The mouse Fas-ligand gene is mutated in gld mice and is part of a TNF family gene cluster.
Article Snippet: David H. Lynch,’ Mark L. Watson,z Mark R. Aiderson, Peter R. Baum,’ Robert E. Miller,’ Teresa Tough,3 Marylou Gibson,5 Terri Davis-SmitN, Craig A. SmitN, Kent Hunter’, Deepti Bhat,2 Wenie Din,’ Raymond G. Goodwin,’ and Michael F. Seidi+ ‘Department of immunobioiogy lmmunex Research and Development Corporation 51 University Street Seattle, Washington 98101 Tithe Autoimmune Genetics Center Division of Rheumatology Departments of Microbiology and Medicine Duke University Durham, North Carolina 27710 3Department of Cellular Immunology 4Department of Molecular Biology 5Department of Mammalian Cell Development 6Department of Biochemistry lmmunex Research and Development Corporation 51 University Street Seattle, Washington 98101 ‘Center for Cancer Research Massachusetts Institute of Technology Cambridge, Massachusetts 02139

Affinity Purification:

Article Title: Local VE-cadherin mechanotransduction triggers long-ranged remodeling of endothelial monolayers
Article Snippet: Soluble VE-cadherin-Fc protein was harvested from a stably transfected CHO cell clone that was selected and cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% (v/v) fetal bovine serum (FBS) and 800 μg/ml G418 (VWR International). .. The soluble VE-cadherin-Fc–His 6 protein (VE-cadherin-Fc) was affinity purified with a Ni-NTA column, followed by a protein-A Affigel column (Bio-Rad, Hercules, CA). ..

Isolation:

Article Title: Use of antisperm antibodies in differential display Western blotting to identify sperm proteins important in fertility.
Article Snippet: .. Isolation of immunoglobulin G from normal sperm extracts Normal sperm extracts, prepared from 200 106 sperm, pooled from six donors as above, were passed through a 5 ml protein A-AffiGel® column (Biorad, Auckland, New Zealand) that had been equilibrated with PBS, pH 7.4. ..